Principles and technical skills fundamental to the culture of animal cells. Introduction to the techniques of sub-culturing, establishing primary cultures, karyotyping, cloning, growth curves, and cryopreservation. Techniques involving culturing mammalian cells in bioreactors will be introduced. Application of cell culture to contemporary research in biotechnology through independent projects.

The graduate seminar in biotechnology will run each semester with different topics. 1) Cutting-edge issues facing the biotech industry will be encountered through case studies in order to apply what is being learner in other courses, hone communication skills, and stay up to date in the fields. 2) Instrumentation and technologies utilized in the biotechnology industry will be described through lectures, readings, and site visits to nearby facilities.

Fundamental relationships of traffic speed, density, and flow applied to public and private modes of transport. Principles of demand forecasting and urban systems planning. Prereq: permission.

A thorough examination of the problems which exist in hazardous and solid waste management will be presented in terms of the current regulations and engineering approaches used to develop solutions. Topics will include risk-based decision making, transport and fate of contaminants, and the fundamental physical, chemical and biological concepts which make up the basis for technological solutions to these waste management problems. Case studies will be used throughout the course to highlight key concepts and provide real-world examples.