Introduction to culture and phenotyping of mammalian cells (cell line models), with applications to bioengineering and biomedical sciences. Skills, techniques, and knowledge covered include sterile technique, cell culture, cell line models, cell proliferation, cell survival, cell migration, cell adhesion, and drug response. Inquiry-based team projects investigate cell proliferation, cell death, transfection, flow cytometry, 3D scaffolds, or cell imaging.